Strains are the root of production, and seed production is the basic link in the production of edible fungi. Edible mushroom cultivation, its success or failure and the level of production, quality is good or bad, are related to the quality of the bacteria. High-quality, high-yield strains not only grow rapidly, but also have strong antibacterial properties, short production cycles, and high yields. Now we introduce our own seed production methods as follows: 1. The parent species used in the production of parent species, whether introduced from outside or separated from the species, should generally be controlled within three generations of the transfer of parent species. The bacteria age should be suitable. The characteristics of high-quality mothers: white, dense, thick, neat, hyphae, no pigment, less aerial hyphae, mushroom flavor. 1.1 200 grams of potato seed medium formulation, 20 grams of glucose, ZO grams of agar, 1 kilogram of potassium dihydrogen phosphate, 0.50 grams of magnesium sulfate, and 1000 ml of water with a pH of 5.50 to 6.50. 1.2 Preparation of medium: Select fresh potatoes that are not germinated, disease-free or green, wash and peel, weigh 200g, cut into small pieces, and put them in a beaker (measurement cup). O into 1000ml of clean water, heat and boil. Maintain 20 to 30 minutes until the potato crisps are not rotted. Add a little stirring during the heating process. Then use 3 layers of gauze to filter. Take the filtrate and make up to 1000 ml. Add agar to the potato sap and continue heating and stirring to agar. Dissolve, and finally add glucose, potassium dihydrogen phosphate, magnesium sulfate, make up to 100 ml with water, measure with a pH test paper and adjust the pH. In normal operation, the pH value is often within the required range and the measurement can often be ignored. Dispense in test tubes at a height of 1/8 to 1/5 of the height of the test tube, add a tampon, wrap it, and sterilize it in a pressure cooker. Point the pointer to 0.0 4 M PA for cold air and then pressurize it to 0.105 MpA. For 30 minutes, naturally cool the pan to about 60°C and put it on a slant. 1.3 Inoculation culture According to the conventional method after inoculation, the parent species is placed in the culture room, the temperature is 23-25° C., and the air relative humidity is about 65% cultured for 7-10 days. The mycelium is covered with the medium slope and can be used to connect the original species. 2. The selection and preparation of raw materials for seed production The selection and preparation of raw materials for seed production has a direct relationship with the quality of strains produced and the effects of their use. The commonly used raw materials are wheat (corn X cottonseed husks, etc.) 2.1 Original species formula 2.1.1: Wheat (corn ): 95%; gypsum powder: 2O% superphosphate: 2O%; urea: 0.50%; white sugar: 0.50%; pH: 5.50 ~ 6.50 2.1.2 seed husk: 87%; bran: 10%; sucrose White sugar: 0.50%; Gypsum powder: 1%; Superphosphate: 1%; Urea: 0.50%; Water addition: 120% - 130% 2.2 Preparation of medium 2.2.1 Screening of JJ and wheat (maize) Quantity, set in the water soak 2 hours, then stir in boiling water while stirring, check the degree of cooking at any time, especially after cooking 15 minutes more to check, until the wheat (maize) no white heart, cooked without When it is rotten (can't bloom), remove it immediately and put it on a nylon cloth or a clean concrete floor to dry it. When there is no excess moisture on the surface of the corn (corn), add gypsum powder, superphosphate, etc., stir well and bottling (use 500g can or wine bottle X bottle plus two newspapers, covered with a high temperature plastic, with a rubber band ( Bicycle waste tube cut into pieces, each treaty can be cut 400) When the pressure required sterilization 0.105MpA, maintain 2 to 3, when the sterilization should pay attention to the time of cold air, pay attention not to overpressure, sterilization time must be accurate 2.2.2 Weigh the raw materials first, dissolve the sugar, gypsum powder, urea, superphosphate, etc., and then make the mother liquor, dilute c according to the required water, then wet the cotton seed husks, stack them for 2 to 3 hours, and stir. Bran, mix well, squeeze the culture material by hand, and use the water in the fingers to make extravasation without dropping downwards.The bottle is compacted slightly, and the plugging and high pressure method is the same as above 2.2.3 Inoculum culture: A bacteria The culture medium is inoculated when the pan is cooled to room temperature, and each test tube can be connected to 5 to 6 bottles of the original species.The culture conditions are the same as the parent species, and generally the bottle can be filled for 20 to 25 days.Note: The bottle is cultured to 10~ Shake the bottle for 12 days, and then culture.The quality of the original species is characterized by white and neat mycelia, and the wall and surface of the bottle is covered with hyphae and mushrooms.3.Cultivated species production 3.1 Medium preparation and formulation with the original system 3.2 Inoculation Culture First, use 75% alcohol or 3% to wash your hands and the original bottle Toxic, then use a sterilized inoculum shovel (锄 翻 翻 翻 loose original species, in the inoculation box (above the clean bench) above the flame of the alcohol lamp, pour a little seed into the sterilized bottle, each bottle of 20 ~ 25 bottles, the culture conditions are the same as the original species, usually 20 to 25 days can be overgrown, and should be sowed immediately after being grown.If not used for a while, it can be stored and dried at 10 to 14 degrees Celsius for no more than 10 days; 2 to 4 degrees Celsius When stored for no more than 20 days, the cultivar preserved at low temperature is returned to normal temperature for 1 to 2 days before use.. 4. Summary: The above seed production method has the following advantages 4.1 Use of wheat (maize) bacteria Species: The production method is simple, labor-saving, strain quality is good, shake the bottle 1 or 2 times during the cultivation, the mycelia and the wheat grains are evenly mixed, and the mycelium grows up and down. 4.2 The use of cottonseed husk as raw material has a low cost: about 5 bottles per 500g of cottonseed hull can be quickly grown and the quality of the strains is good. Compared with wheat grains, the operation is slightly troublesome. When the original species is expanded and cultivated, the inoculation shovel (锄) is required to complete the cultivation. 4.3 Short production cycle of strains: Generally only 50 to 55 days from the isolation of the parent to the cultivar. 4.4 The amount of bacteria used in the province: generally 1 bottle of cultivar can inoculate 5 to 6 bags of 23 to 50 cm size. 4.5 Fast germination: After 35 to 50 days in the cultivating seed bag, the full line can be covered.
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