Under natural conditions, Koi carcasses are spawned and spawning is not synchronized, and the hatching rate and seedling survival rate of fertilized eggs are extremely low, resulting in insufficient seedlings for large-scale artificial breeding. After two years of exploration and research, we have solved this problem by artificially urging production and strengthening seed cultivation. The relevant technologies are summarized below.
1 artificial labor
1.1 The selection of broodstock is generally performed before the winter in pools and ponds. The selection of Koi carcasses that are robust, free of injury, scales, and bright-colored as their parents can also be based on the color characteristics of various Koi fishes. Males require more than 2 years of age, females require more than 3 instars, male and female ratio 1;2. When the operation is light, the fish should not be injured. The selected color should be immediately returned to the pro-fish pond. It should not be trapped. The female and male progeny should be kept separately to prevent miscarriage.
1.2 Breeding of broodstock Once the broodstock is selected, it begins to grow. The stocking density of broodstock is controlled within 150Lg per mu. The amount of investment is 1% to 2% of the fish's body weight every day in the winter sky. The day is particularly cold or rainy or snowy days. Feeding or stopping feeding is delayed; after the Spring Festival, the amount of feed at the end of February increases to 4% of the fish's body weight. ~ 5%, part of the high-protein turtle material and the necessary barley or wheat shoots are added to feed to ensure the development of gonads. In late March. Guarantee to flush for more than half an hour every day, while preventing the floating head phenomenon of broodstock.
1.3 Preparation of spawning ponds Spawning ponds are required to have no weeds in their vicinity. Before March 25, water is drained for proper disinfection. When disinfecting, all debris in the pool must be removed. In particular, the weeds must be cleaned to keep the pond clean, which is conducive to the placement of the ovaries, and it can also prevent the eggs from being produced on the sundries and cause unnecessary losses. .
1.4 Preparation of fish nests There are various types of fish nests, but palms are preferred. Palms should be washed, softened, and smashed. Each 2 to 3 pieces must be fastened. Prevent egg shedding. After being tied up, it will be exposed to the sun. On March 25th, about 20 10-6 concentration of malachite green will be soaked and disinfected. Soaking time in 20 minutes, soak after drying, cool, ready to need.
1.5 Artificial Fertility When the water temperature stabilizes at about 20°C for 3 consecutive days, observe the fish's activity and find out that the fish is surrounded by a large amount of water and the water is thrown up. This corresponds to the immediate dry pool preparation for production. In combination with the characteristics of carp, we used PG, DON and LRHA-2 for injection. A one-time thoracic injection method was used. The injection dose was PGlmg, DON 1 mg, and LRHA 210 μg per kg of female fish. The injection volume was 2 ml and the male was halved. The injection time is best controlled between 13:00 and 14:00, which is conducive to estrus at night and spawning.
1.6 After hanging the fish nest, the male and female broodstock of the broodstock are injected into the spawning pool and stimulated by microfluidic water. And timely into the fish nest, each fish nest is inserted with bamboo sticks at about 1 meter away from the edge of the pool, the ovaries can not enter the water can not be too deep, fish nest spacing 3-5 meters. It is beneficial for brooders to find nests spawning in time.
2 seed cultivation
2.1 Preparation of Seed Pool The seedling pool is preferably 1-2 mu, which is conducive to feeding management. The dry pool is disinfected around March 25, and 20 kg of bleach is used to completely kill bacteria and harmful organisms. Three days after clearing, the water is injected, and the water injection depth is controlled at about 70 cm. It should not be too deep. Mesh cloth should be used for water injection to prevent the entrance of harmful organisms. After entering the water, it is not appropriate to immediately apply fertilizer, because most of the fish ponds are also used as hatching pools. It is not easy to increase the hatching rate due to excessive water quality.
2.2 Incubation of Fertilized Eggs We adopt the method of hydrostatic incubation and treat the seedlings as a hatchery. The fish nest with eggs is removed from the spawning pond in time, and soaked in malachite green liquor at a concentration of 10 10-6 for 3 to 5 minutes, and then the seedling pool is hatched (conditional can be hatched in a cement pool, etc. After the seedlings are put into the seedling pool, the effect will be better.) The fish nest is not submerged in water, and the distance between the 30-40 cm fish nests is controlled to be about 30 cm away from the water surface. Malachite green liquor with a concentration of 10 10-6 is used every day to spit and disinfect the fish nest and its surroundings to prevent the generation of molds until the fish eggs. Show your eye so far. Oviposition density can be controlled at about 200,000 grains per acre.
2.3 Seedlings cultivate fish nests and start pouring soymilk 3 days later. Soymilk should be ground and thin, and Quanchiposa should be used. The amount of feed should be 0.5kg soybeans per acre of water and 1kg after 1 week, after 10 days. On the basis of feeding soymilk, the cabbage cake was fed once and the amount of water fed per acre was 5kg.
2.4 seed oil management adhere to the daily patrol pool, observe the growth of fish, the transparency of the water control in 25-30cm, every 4 - 5 days, add water, each time the amount of water control in about 10cm. Koi has a clustering phenomenon, month. Bright colors. It is vulnerable to birds such as gulls, and it is necessary to do a good job of driving away birds to prevent unnecessary losses.
2.5 Pull net selection When the fish body reaches 3cm in length, the net can be selected, and the operation is strictly performed according to the color and color requirements of each species. Remove individuals with bad colors and colors. The selected koi can be sold directly, or it can be kept in separate ponds and sold after reaching certain specifications.
3 Summary
It has been proved that the artificial production of Koi can promote the relative spawning of Koi to form a certain batch, which is conducive to seed breeding and large-scale adult fish breeding. It is an effective way to solve the hard-to-find Koi seedlings.
1 artificial labor
1.1 The selection of broodstock is generally performed before the winter in pools and ponds. The selection of Koi carcasses that are robust, free of injury, scales, and bright-colored as their parents can also be based on the color characteristics of various Koi fishes. Males require more than 2 years of age, females require more than 3 instars, male and female ratio 1;2. When the operation is light, the fish should not be injured. The selected color should be immediately returned to the pro-fish pond. It should not be trapped. The female and male progeny should be kept separately to prevent miscarriage.
1.2 Breeding of broodstock Once the broodstock is selected, it begins to grow. The stocking density of broodstock is controlled within 150Lg per mu. The amount of investment is 1% to 2% of the fish's body weight every day in the winter sky. The day is particularly cold or rainy or snowy days. Feeding or stopping feeding is delayed; after the Spring Festival, the amount of feed at the end of February increases to 4% of the fish's body weight. ~ 5%, part of the high-protein turtle material and the necessary barley or wheat shoots are added to feed to ensure the development of gonads. In late March. Guarantee to flush for more than half an hour every day, while preventing the floating head phenomenon of broodstock.
1.3 Preparation of spawning ponds Spawning ponds are required to have no weeds in their vicinity. Before March 25, water is drained for proper disinfection. When disinfecting, all debris in the pool must be removed. In particular, the weeds must be cleaned to keep the pond clean, which is conducive to the placement of the ovaries, and it can also prevent the eggs from being produced on the sundries and cause unnecessary losses. .
1.4 Preparation of fish nests There are various types of fish nests, but palms are preferred. Palms should be washed, softened, and smashed. Each 2 to 3 pieces must be fastened. Prevent egg shedding. After being tied up, it will be exposed to the sun. On March 25th, about 20 10-6 concentration of malachite green will be soaked and disinfected. Soaking time in 20 minutes, soak after drying, cool, ready to need.
1.5 Artificial Fertility When the water temperature stabilizes at about 20°C for 3 consecutive days, observe the fish's activity and find out that the fish is surrounded by a large amount of water and the water is thrown up. This corresponds to the immediate dry pool preparation for production. In combination with the characteristics of carp, we used PG, DON and LRHA-2 for injection. A one-time thoracic injection method was used. The injection dose was PGlmg, DON 1 mg, and LRHA 210 μg per kg of female fish. The injection volume was 2 ml and the male was halved. The injection time is best controlled between 13:00 and 14:00, which is conducive to estrus at night and spawning.
1.6 After hanging the fish nest, the male and female broodstock of the broodstock are injected into the spawning pool and stimulated by microfluidic water. And timely into the fish nest, each fish nest is inserted with bamboo sticks at about 1 meter away from the edge of the pool, the ovaries can not enter the water can not be too deep, fish nest spacing 3-5 meters. It is beneficial for brooders to find nests spawning in time.
2 seed cultivation
2.1 Preparation of Seed Pool The seedling pool is preferably 1-2 mu, which is conducive to feeding management. The dry pool is disinfected around March 25, and 20 kg of bleach is used to completely kill bacteria and harmful organisms. Three days after clearing, the water is injected, and the water injection depth is controlled at about 70 cm. It should not be too deep. Mesh cloth should be used for water injection to prevent the entrance of harmful organisms. After entering the water, it is not appropriate to immediately apply fertilizer, because most of the fish ponds are also used as hatching pools. It is not easy to increase the hatching rate due to excessive water quality.
2.2 Incubation of Fertilized Eggs We adopt the method of hydrostatic incubation and treat the seedlings as a hatchery. The fish nest with eggs is removed from the spawning pond in time, and soaked in malachite green liquor at a concentration of 10 10-6 for 3 to 5 minutes, and then the seedling pool is hatched (conditional can be hatched in a cement pool, etc. After the seedlings are put into the seedling pool, the effect will be better.) The fish nest is not submerged in water, and the distance between the 30-40 cm fish nests is controlled to be about 30 cm away from the water surface. Malachite green liquor with a concentration of 10 10-6 is used every day to spit and disinfect the fish nest and its surroundings to prevent the generation of molds until the fish eggs. Show your eye so far. Oviposition density can be controlled at about 200,000 grains per acre.
2.3 Seedlings cultivate fish nests and start pouring soymilk 3 days later. Soymilk should be ground and thin, and Quanchiposa should be used. The amount of feed should be 0.5kg soybeans per acre of water and 1kg after 1 week, after 10 days. On the basis of feeding soymilk, the cabbage cake was fed once and the amount of water fed per acre was 5kg.
2.4 seed oil management adhere to the daily patrol pool, observe the growth of fish, the transparency of the water control in 25-30cm, every 4 - 5 days, add water, each time the amount of water control in about 10cm. Koi has a clustering phenomenon, month. Bright colors. It is vulnerable to birds such as gulls, and it is necessary to do a good job of driving away birds to prevent unnecessary losses.
2.5 Pull net selection When the fish body reaches 3cm in length, the net can be selected, and the operation is strictly performed according to the color and color requirements of each species. Remove individuals with bad colors and colors. The selected koi can be sold directly, or it can be kept in separate ponds and sold after reaching certain specifications.
3 Summary
It has been proved that the artificial production of Koi can promote the relative spawning of Koi to form a certain batch, which is conducive to seed breeding and large-scale adult fish breeding. It is an effective way to solve the hard-to-find Koi seedlings.
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