Friends who use the 100x microscope for the first time may not know much. The eyepieces with a slightly larger magnification are attached to the specimen but only the color of the specimen can be seen. Other details cannot be seen. I don't know how to use it.
In fact, the 100x objective lens needs to be observed by dripping oil. This is called the oil mirror. When observing, you need to drop a drop of pine oil in the observed area, then the microscope first finds the image from low magnification, adjusts the thickness and focus, and then changes it to 100 times one by one.
There is a problem here. The larger the objective lens magnification, the smaller the distance between the objective lens and the specimen: Is it not necessary to use the cedar oil to observe the specimen? In fact, the 100x objective lens is not attached to the specimen, but the distance is too close. The cedar oil is used to make the objective lens and the surface of the object in the same medium, increasing the refractive index.
For example, if I look at a specimen of a leaf, I don't need to stick the cedar oil to the specimen. I can only see a piece of green. The same distance can be seen by dropping the cedar oil. (Transformation question: the original 20x eyepiece 100x objective lens did not see clearly, not because of the distance problem but because of the problem of no phloem, 20x eyepiece 100x objective lens can be seen clearly?)
The fact is this, as mentioned above, the 100x objective lens belongs to the oil mirror, and it is used when there is no oil drop. It can only see the effect in a fuzzy way. Only the oil drops the effect of the objective lens.
When using the oil mirror, add cedar oil to the slide. This is because the magnification of the oil mirror is high, and the lens is very small. When the light passes through the medium object of different density (slide → air → lens), part of the light will be refracted and lost, and the light entering the lens barrel is less, and the field of view is smaller. Dark, the object is unclear. For example, if cedar oil (n=1.515) similar to the refractive index of glass (n=1.52) is dropped between the lens and the slide, the light entering the oil mirror is increased, the brightness of the field of view is enhanced, and the image is clear.
There are many precautions when using oil mirrors.
(1) Before using the oil mirror, it must be observed by low and high magnification, and then the part to be further enlarged is moved to the center of the field of view.
(2) Raise the concentrator to the highest position and open the aperture to the maximum.
(3) Rotate the converter to make the high-power lens leave the light-passing hole, add a drop of cedar oil to the slide to be observed, and then slowly turn the oil mirror. When converting the oil mirror, look at the lens and the slide horizontally from the side. The distance is such that the lens is immersed in the oil without crushing the slide.
(4) Observe the eyepiece with your left eye and slowly turn the fine adjuster until the object is clear.
If there is no object or the target is not ideal, you should re-find it. If you are looking for it outside the fueling area, you should press: low magnification → high magnification → oil mirror procedure. Re-find in the fueling area should be: low magnification → oil mirror procedure, not through high power mirror, so as to avoid oil staining the lens.
(5) After the oil mirror is used, first wipe it with the lens paper, then wipe the lens and the cedar oil on the specimen with a little xylene-coated lens paper, and then wipe it off with a dry mirror paper.
Familiar with the use of a variety of objective lenses in order to effectively observe and measure objects. Using the imported Olympus objective lens, the effect is much better than the national product mirror that you have configured. The objective lens of what grade should be selected should have a certain understanding of the elimination of chromatic aberration according to the experiment and needs of the experiment.
In fact, the 100x objective lens needs to be observed by dripping oil. This is called the oil mirror. When observing, you need to drop a drop of pine oil in the observed area, then the microscope first finds the image from low magnification, adjusts the thickness and focus, and then changes it to 100 times one by one.
There is a problem here. The larger the objective lens magnification, the smaller the distance between the objective lens and the specimen: Is it not necessary to use the cedar oil to observe the specimen? In fact, the 100x objective lens is not attached to the specimen, but the distance is too close. The cedar oil is used to make the objective lens and the surface of the object in the same medium, increasing the refractive index.
For example, if I look at a specimen of a leaf, I don't need to stick the cedar oil to the specimen. I can only see a piece of green. The same distance can be seen by dropping the cedar oil. (Transformation question: the original 20x eyepiece 100x objective lens did not see clearly, not because of the distance problem but because of the problem of no phloem, 20x eyepiece 100x objective lens can be seen clearly?)
The fact is this, as mentioned above, the 100x objective lens belongs to the oil mirror, and it is used when there is no oil drop. It can only see the effect in a fuzzy way. Only the oil drops the effect of the objective lens.
When using the oil mirror, add cedar oil to the slide. This is because the magnification of the oil mirror is high, and the lens is very small. When the light passes through the medium object of different density (slide → air → lens), part of the light will be refracted and lost, and the light entering the lens barrel is less, and the field of view is smaller. Dark, the object is unclear. For example, if cedar oil (n=1.515) similar to the refractive index of glass (n=1.52) is dropped between the lens and the slide, the light entering the oil mirror is increased, the brightness of the field of view is enhanced, and the image is clear.
There are many precautions when using oil mirrors.
(1) Before using the oil mirror, it must be observed by low and high magnification, and then the part to be further enlarged is moved to the center of the field of view.
(2) Raise the concentrator to the highest position and open the aperture to the maximum.
(3) Rotate the converter to make the high-power lens leave the light-passing hole, add a drop of cedar oil to the slide to be observed, and then slowly turn the oil mirror. When converting the oil mirror, look at the lens and the slide horizontally from the side. The distance is such that the lens is immersed in the oil without crushing the slide.
(4) Observe the eyepiece with your left eye and slowly turn the fine adjuster until the object is clear.
If there is no object or the target is not ideal, you should re-find it. If you are looking for it outside the fueling area, you should press: low magnification → high magnification → oil mirror procedure. Re-find in the fueling area should be: low magnification → oil mirror procedure, not through high power mirror, so as to avoid oil staining the lens.
(5) After the oil mirror is used, first wipe it with the lens paper, then wipe the lens and the cedar oil on the specimen with a little xylene-coated lens paper, and then wipe it off with a dry mirror paper.
Familiar with the use of a variety of objective lenses in order to effectively observe and measure objects. Using the imported Olympus objective lens, the effect is much better than the national product mirror that you have configured. The objective lens of what grade should be selected should have a certain understanding of the elimination of chromatic aberration according to the experiment and needs of the experiment.
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