In-tank preparation and cleaning and sterilization method of fermenter equipment

The fermenter equipment adopts the internal circulation method, and uses a stirring paddle to disperse and break the air bubbles, which has a high dissolved oxygen rate and a good mixing effect. The tank body is made of SUS304 or 316L imported stainless steel, and the tank is equipped with automatic spray cleaning head to ensure the production process meets GMP requirements. Fermenters are widely used in dairy, beverage, bioengineering, pharmaceutical, fine chemical and other industries. In recent years, with the continuous advancement of science and technology, the fermentation industry, which is related to the national economy and the people's livelihood, has achieved rapid development, and the fermenter equipment has also been updated from the traditional to the modernization direction.

The fermenter structure for anaerobic fermentation can be simpler. The requirements for this type of fermenter are: can be closed; can withstand a certain pressure; have cooling equipment; minimize the device in the tank, eliminate dead angles, and facilitate cleaning and sterilization. Alcohol and beer are all anaerobic fermentation products. Because the fermenter does not need to pass expensive sterile air, it is much simpler than aerobic fermentation equipment when the equipment is enlarged, manufactured and operated.

Fermentation tanks used for aerobic fermentation require a large amount of sterile air to be continuously supplied into the tank, and in order to consider the utilization rate of the air, the structure of the fermenter is complicated, and a mechanically stirred fermenter and bubbling are commonly used. Fermenter and airlift fermenter.

The structure of the fermenter is relatively complicated, and the internal corner angle is not easy to be disinfected. The general fermenter has an automatic cleaning device, and is completely sterilized after cleaning.

1. Fermentation tank Before the medium is sterilized, the sub-air filter connected to the tank should usually be steam sterilized and blown dry with air. When the tank is sterilized, the sewage in the feed line is first drained, and then the prepared medium is pumped into the fermenter (seed tank or tank), and the stirrer is activated to sterilize. Before the sterilization, the exhaust valves are opened, and the steam is introduced into the jacket or the coil for preheating. When the temperature of the tank rises to 80-90 ° C, the exhaust valve is gradually closed. Then, the steam is directly introduced into the tank from the air inlet, the discharge port and the sampling port (if there is a sight tank, the steam is also introduced), so that the tank temperature rises to 118-120 ° C, and the tank pressure is maintained at 0.09 - 0.1 Mpa (gauge pressure), and kept for about 30min. The intake of each road should be unblocked to prevent short-circuit countercurrent, and the liquid in the tank should be fiercely turned; the exhaust of each road should be smooth, but the amount of steam should not be too large to save gas consumption. In the heat preservation stage, all pipes and sight glass tubes imported below the liquid level of the medium should be steamed; those who open above the liquid level should be exhausted. Regardless of how the piping is connected to the tank, the principle of “no advances and no ones” should be followed in the actual elimination. This will ensure complete sterilization and no dead ends. After the end of the heat preservation, the exhaust steam and steam inlet valves are closed in turn. After the pressure in the tank is lower than the air pressure, the sterile air is introduced into the tank, and the cooling water is cooled in the jacket or the coil to lower the temperature of the medium. At the desired temperature, the next fermentation and culture are carried out. Before introducing sterile air, it should be noted that the tank pressure must be lower than the filter pressure, otherwise the material will fall into the filter, the consequences are serious! When sterilizing, the total steam pipeline pressure requirement is not less than 0.3 - 0.35Mpa, and the use pressure is not less than 0.2Mpa.

2. Fermentation tank empty tank sterilization (empty) is the sterilization of the ferment tank. Generally, the tank is maintained at 0.15-0.2Mpa, the tank temperature is 125-130°C, and maintained at 30-45min; the total steam pressure is required to be no less than 0.3-0.35Mpa, and the steam pressure is not less than 0.25-0.3MPa. .