Human fibroblast growth factor 21 (FGF-21) ELISA kit
All the elisa kits of Shanghai Keshun Biotechnology Co., Ltd. provide the free ELISA test kit for the special test laboratory :
(1) Solid phase of an antigen or antibody and enzymatic labeling of an antigen or an antibody.
(2) The antigen or antibody bound to the surface of the solid support still retains its immunological activity.
(3) The enzyme-labeled antigen or antibody retains both its immunological activity and the activity of the enzyme.
(4) The test specimen reacts with an antigen or antibody on the surface of the solid phase carrier. Further, an enzyme-labeled antigen or antibody is added, and is also bound to the solid phase carrier by a reaction.
(5) At this time, the amount of enzyme on the solid phase is proportional to the amount of the substance to be tested in the specimen.
(6) After adding the substrate of the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the substance to be tested in the sample, so that qualitative or quantitative analysis can be performed according to the depth of the color. The assay has high sensitivity (pg-ng/ml level) and good repeatability. A highly sensitive test technique that combines the specific reaction of antigens and antibodies with the efficient catalytic action of enzymes on substrates based on immunological reactions.
The human fibroblast growth factor 21 (FGF-21) ELISA kit is a sensitive ELISA that combines the specific reaction of antigen and antibody with the efficient catalytic action of the enzyme on the substrate. Very high test technique.
Precautions:
1. The kits produced by different manufacturers are slightly different according to the production process and operation method. It is necessary to strictly operate according to the kits used, otherwise the uncertainty of the test results is easy to occur.
2. If different components of different batches of human ELISA kits (A solution or enzyme working solution), or different components of different reagents are used in cross-over, there may be cases of light color development or high background, flower plate and the like.
3. The error of the reaction time. When a large number of specimens are made, the first hole and the last hole and some special specimens may have a large influence.
4. The fluctuation of the specimen near the critical value is a normal phenomenon, and any reagent is inevitable. You can consider making the specimen an odd number of retests.
5. The sample volume error during sample loading has little effect on the yin or very positive specimens, but it has a great influence on the specimens near the threshold. It is recommended to proofread the sampler.
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The human fibroblast growth factor 21 (FGF-21) ELISA kit is to be tested . The spot product will be sent to the customer within one week from the date of receipt of the customer's specimen! Years of professional services, quality assurance, escort your research, can provide free testing services, look forward to your call!
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