product description
| Â English name | Â Human PRL (Prolactin) CLIA Kit | ||
|  Chinese name  |  Human Prolactin (PRL) Chemiluminescence Immunoassay Kit  | ||
| Â Item number | Â E-CL-H0133c | Â species | Â Human / person |
| Â specification | Â 96T/Kit (8*12 strips) 48T/Kit (8*6 strips) | ||
|  Detection method  |  Double antibody sandwich method  | ||
| Â examination range | Â 1.563~100ng/mL | Â Sensitivity | Â 0.938ng/mL |
Fundamental
This kit uses a double antibody sandwich method. The anti-Human PRL antibody is coated on the microtiter plate, and the PRL in the specimen or standard is bound to the coated antibody, and the free component is washed away. Biotinylated anti-Human PRL antibody and horseradish peroxidase-labeled avidin were sequentially added. The anti-Human PRL antibody binds to Human PRL bound to the coated antibody, and biotin binds to avidin to form an immune complex, and the free component is washed away. The luminescent substrate mixture was added, the luminescent substrate was fluoresced under the catalysis of horseradish peroxidase, and the chemiluminescence value (CL) was measured by a chemiluminescence immunoassay. The positive relationship between the PRL concentration and the chemiluminescence value was plotted. The standard curve is used to determine the concentration of PRL in the specimen.
Steps
1. Add 100 μL of standard or sample to each well and incubate at 37 ° C for 90 minutes.
2. Pour the liquid in the well, pat dry, add 100 μL of biotinylated antibody working solution, incubate at 37 ° C for 60 minutes.
3. Wash 3 times
4. Add 100 μL of enzyme conjugate working solution and incubate at 37 ° C for 30 minutes.
5. Wash 5 times
6. Add 100 μL of substrate solution and incubate for 5 minutes at 37 °C
7. Readings
8. Calculation of results
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