Cell culture is the separation of tissue fragments into individual cells by enzymatic digestion, and the cell suspension is made from the medium, and the cells are allowed to grow and reproduce under suitable conditions in vitro, and retain certain structural and functional characteristics. The main difference between cell culture and tissue culture and organ culture is that the original cultured objects are different. The cell culture uses a single cell suspension, and the tissue culture uses a tissue block (0.5 to 1 mm 3 ) or a sheet (0.2 mm thick), and the organ culture uses a part of the organ primordial or an organ or an entire organ. In tissue culture, cells move out of the tissue block and grow. There are always movements (motions) or other changes in the growth process, which makes it difficult for the cultured tissue to maintain its original structure and function for a long time. The longer the culture time, the more likely it is to change, and as a result, a single type of cell is often preserved and eventually becomes a cell culture. In cell culture, cell life activities, like cells in the body, are still interdependent and present a certain tissue specificity, so there is no strict distinction between tissue culture and cell culture.
Cell culture technology is a commonly used research method in life sciences. This method can eliminate the influence of neurohumoral factors and the interference of liver and kidney detoxification functions, and observe the direct effects of certain factors or drugs on cultured cells. Pure culture of a certain type of cell can be obtained by experiment. For example, myocardial cells account for about 50% of myocardial cells, and non-cardiomyocytes account for 50%. In the isolated and isolated cardiomyocyte suspensions, cardiomyocytes can reach more than 95%. Thus, the primary culture of cardiomyocytes is basically free of other cells. Interference. The dynamic process of culturing cell life activities can be directly observed in cell culture experiments; some life phenomena that are not visible to the naked eye can be found by timed microphotographing; electron microscopy, isotope labeling, radioimmunoassay and immunohistochemistry can also be used. Etc. to study the cell morphology and distribution of intracellular chemicals. In addition, research costs can be saved. As in some studies, the conclusions obtained with 100 animals were the same as those obtained with 100 coverslips or dozens of culture flasks, while cell culture costs compared to large-scale animal feeding. Be small.
However, there are also shortcomings in the cell culture method: the cultured cells lose the restriction of the cells in the body and the overall regulation, and the cell morphology and function will change to some extent. Culture methods and experimental reagents have certain effects on cell morphology and function. For example, trypsin can destroy cell surface receptors, enzymes, antigens, and the like. Long-term in vitro culture of cells, due to repeated passage, cryopreservation and manipulation, may cause chromosomal aneuploidy changes, characterized by immortalization or canceration. 》》》》》 More specific information you can enter the Shanghai Baiwo Biotechnology Co., Ltd. official website
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