The structure principle, operation and maintenance of the inoculation ring sterilizer!

The structure principle, operation and maintenance of the inoculation ring sterilizer!

I. Introduction <br> flame-sterilized classical microbiological treatment, but in the course of an aerosol is generated, and a biological safety cabinet airflow disorder can, therefore, not suitable for flame-sterilized using a biological safety cabinet . The electronic sterilizer not only replaces the traditional alcohol lamp, Bunsen burner, etc., but also has the advantages unmatched by the traditional flame sterilization tool, and is very suitable for use in laboratory and biological safety cabinets.
The electronic flame sterilizer is compact and equipped with a complete safety protection device for extremely convenient operation. Therefore, the World Health Organization (WHO) and major biosafety cabinet manufacturers recommend that electronic sterilization be used instead of flame disinfection in biosafety cabinets.

Second, the basic structure and principle <br> Electronic sterilizer according to the different principles, can be divided into infrared heating sterilizer, electronic flame sterilizer and resistance heating sterilizer.
1. Infrared heating sterilizer <br> Infrared heating type inoculation ring sterilizer, consisting of outer casing, ceramic tube, electric heating wire, high temperature quartz tube and temperature controller. The ceramic tube has a cylindrical shape, one end is located on the top or side of the outer casing, and the other end is located in the outer casing. The outer end of the ceramic tube is respectively provided with an outer furnace cover and an inner furnace cover, and a temperature sensor is installed in the small circular hole of the inner furnace cover. The outside of the ceramic tube is wrapped with a heating wire, and the outer surface of the heating wire is covered with mica tape, and the outer surface of the mica tape is covered with high-temperature aviation cotton. A temperature controller and a display 3 infrared heating sterilizer are mounted on the upper rear portion of the outer casing; a temperature insulation plate is arranged between the temperature controller and the ceramic tube. The furnace tube is equipped with a high temperature quartz tube to ensure that the sterilization environment is both hygienic and safe.
2. Electronic Fire Starter <br> Electronic fire starter has been widely used in laboratories in developed countries such as Europe and America. The sterilizer adopts a powerful microprocessor control pair, and uses an infrared sensor ignition switch. When the microprocessor receives the ignition request, it issues an instruction to open the solenoid valve, and emits infrared heat at the top of the burner for sterilization. When the temperature is too high, the microprocessor closes the solenoid valve and the sterilizer stops working. The advent of electronic flame sterilizers has greatly improved the safety issues associated with conventional laboratory flame sterilization or burning devices.
3. Resistance heating sterilizer <br> Resistance heating sterilization technology is also called ohmic sterilization. It is a kind of small cross-section with high electric resistance of platinum wire inoculated with ring, and the heat generated inside the inoculating ring is generated by the current. A sterilization technique that achieves sterilization purposes. The resistance heating sterilizer is relatively simple in construction, and mainly provides positive and negative poles for sterilization. Because it is small in size and does not consume oxygen, it is generally used in anaerobic incubators.

Third, the application operation
1. Infrared heating sterilizer
(1) Turn on the power and turn on the switch. For temperature control, the temperature can be preset. Then preheat to the desired temperature, or wait until the sterilizer lumen turns red.
(2) Put the front end of the inoculation ring into the inner cavity. If it turns red, the instrument can work normally.
(3) After the experiment is terminated, turn off the power of the electric sterilizer.
2. Electronic flame sterilizer
(1) After turning on the power, turn on the infrared sensor ignition switch. When the microprocessor receives the ignition request, it will issue a command to open the solenoid valve and emit infrared heat energy at the top of the burner.
(2) Sterilization and sterilization, if the temperature is too high, the microprocessor immediately closes the solenoid valve and the sterilizer stops working.
(3) If there is tempering when the instrument is working, adjust the air knob to reduce the air entering until the tempering is eliminated.
(4) After the experiment is finished, turn off the infrared sensor ignition switch and disconnect the power supply.
(5) After the instrument is used up, remember to turn off the gas bottle switch and pull the instrument power switch to the OFF position.
3. Resistance heating sterilizer
(1) Turn on the power and turn on the switch.
(2) Handle the inoculation needle with the insulating handle, put the platinum wire on the positive and negative power supply column, the platinum wire turns red instantly, and immediately take the inoculation ring to inoculate.
(3) After the experiment is terminated, turn off the power of the electric sterilizer.

Fourth, use and maintenance 1. When using the electronic sterilizer must be strictly in accordance with the instructions, to avoid burns and electric shock.
2. When the instrument does not work, the fuse may be blown due to prolonged use, and the fuse needs to be replaced.
3. Use an electronic flame sterilizer to keep the working environment well ventilated. Do not place flammable objects nearby when the instrument is working.
4. The burner of the electronic flame sterilizer works at a high temperature. Do not touch the burner to avoid burns. Do not touch the burner immediately after use.
5. Infrared heating sterilizer will cause some ash accumulation in the inner cavity due to long-term use, and it needs to be cleaned at the right time. Some electronic flame sterilizers use charging or using batteries, so charge or replace batteries regularly. The positive and negative motor column of the resistance heating sterilizer will form an insulating layer on the surface due to long-term use, so it needs to be wiped with fine sandpaper; if it can't be solved, the electrode column can be replaced.
6. After the experiment is finished, turn off the power to the electric sterilizer in time.
In summary, the correct use of laboratory inoculation ring sterilizers is an important part of a laboratory safety practice. Whether using traditional alcohol lamps and Bunsen burners or electronic sterilizers, the operation of the relevant devices must be strictly followed. In order to fully and effectively play its role in routine experimental operations.

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