Scientists have successfully developed individualized functional insulin-producing cells
April 20, 2016 Source: Bio Valley
Window._bd_share_config={ "common":{ "bdSnsKey":{ },"bdText":"","bdMini":"2","bdMiniList":false,"bdPic":"","bdStyle":" 0","bdSize":"16"},"share":{ }};with(document)0[(getElementsByTagName('head')[0]||body).appendChild(createElement('script')) .src='http://bdimg.share.baidu.com/static/api/js/share.js?v=89860593.js?cdnversion='+~(-new Date()/36e5)];Recently, a research report published in the international magazine Cell Metabolism , researchers from the Salk Institute have made major research breakthroughs, they have developed alternative cells for diabetic patients; the researchers found a The hidden energy switch, when "tap" the switch, activates pancreatic cells to respond to glucose, and the significance of the study is that scientists can develop hundreds of millions of human beta cells in the laboratory to relieve diabetes. Symptoms of the mouse.
For more than a decade, scientists around the world have been working on how to treat type 1 and type 2 diabetes. Although they have developed cells that produce insulin in culture dishes, they still cannot respond to glucose. Researcher Ronald Evans In other words, we found that the missing energy switches needed to produce powerful and functional human beta cells, potentially providing potential help in treating human diabetes.
Scientists first used special techniques to generate induced pluripotent cells (iPSCs), which produced pre-beta cells, which produced non-functional insulins; researchers Evans said that pancreatic beta cells must do two Only when things can work effectively, that is, react to glucose and produce insulin, no one can clearly clarify how to use diabetic patients to make pancreatic cells.
In the article, the researchers studied the basic biological mechanisms of beta cells and discovered multiple molecular switches (transcription factors) that control the transition of pancreatic beta cells to functional states, and the researchers discovered a name. A protein switch that is ERR-γ, which plays an important role in waking up sleeping β-like cells to respond to glucose and release insulin. When the researchers added ERR-γ to the beta cells of pre-diabetic patients in a culture dish, they successfully developed beta cells with glucose sensitivity, which was subsequently removed by removing ERR-γ from the animal's body. The effect disappears, indicating that ERR-γ is the major regulator of pancreatic beta cell maturation.
But can these pancreatic beta cells successfully treat diabetes? The researchers said that when transplanting mature β cells into the body of mice with type 1 diabetes, the symptoms of diabetic mice can be effectively restored, which may also play a role in promoting clinical treatment. Diabetes is diagnosed. Patients may be able to use this therapy to treat the disease; the researchers hope that later clinical trials will be conducted to confirm the effectiveness of this therapy in the treatment of diabetes.
Principle
The test utilizes antibodies including a monoclonal LH-β antibody and goat anti-rat IgG on the
nitrocellulose membrane with colloidal gold marked anti-LH-α monoclonal antibody as an mark
tracer. The reagent is used to detect the LH in urine according to the principle of double antibody
sandwich method and gold immunochromatography assay.
Main components
The testing kit is in the form of strip, cassette, and midstream. Basic components: Sample pad,
colloidal gold marked pad, nitrocellulose membrane, absorbent paper and PVC board. Colloidal
gold marked pad coated with LH-α monoclonal antibody, nitrocellulose membrane coated with
LH-β monoclonal antibody,control line coated with goat anti-rat IgG.
Result Judgment
POSITIVE: Two distinct red lines appear. If the color of testing line is stronger than or equal to the
control line, it demonstrates that the ovulation is about to occur in the next 24 to 48 hours.
NEGATIVE: One red line appears in the control region(C). Or both red line appear with the color of
testing line is weaker than the control line, it demonstrates that the ovulation period does not
arrive yet.
INVALID: No red lines appear or control line fails to appear, indicating that the operator error or
reagent failure. Verify the test procedure and repeat the test with a new testing device.
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