1. Treatment methods for bacteria in the fermenter seed culture period
1. Once the fermenter seed is found to be contaminated by bacteria, the seed can no longer be connected to the fermenter for fermentation. It should be discarded after sterilization, and the seed fermenter and pipeline should be carefully inspected and thoroughly sterilized.
2. Using the spare seed, choose to grow the seed that produces the non-stained bacteria into the fermenter and continue the fermentation production.
3. If there is no spare seed, the fermentation broth in a fermenter of appropriate age can be selected as the seed, and the “reverse seeding†treatment is carried out, and the fermentation is carried out in a fresh medium to ensure the normal fermentation production.
Second, the fermenter in the pre-fermentation period of bacterial treatment
1. After the fermenting tank is infected in the early stage of fermentation, if the content of carbon and nitrogen in the medium is relatively high, the fermentation is terminated, the medium is heated to the specified temperature, and the sterilization is further processed, and then the seed is transplanted. Fermentation
2. If the bacteria have caused great harm at this time, the consumption of carbon and nitrogen sources in the medium has been relatively high, then some of the liquid can be drained, fresh medium is added, and after sterilization, Re-inoculation for fermentation.
3. It can also be treated by means of cooling culture, adjusting pH value, adjusting feeding amount and supplementing culture medium.
Third, the fermentation tank in the fermentation, in the late stage of bacterial treatment
1. Fermentation tanks may be added with appropriate fungicides or antibiotics and normal fermentation broth to inhibit the growth rate of the bacteria when the bacteria are slightly contaminated in the early or late stage of fermentation or in the early stage of fermentation. Other measures such as temperature, reduced ventilation, stop stirring, and a small amount of sugar are processed.
2. If the product metabolism of the fermentation process has reached a certain level, if the content of the product reaches a certain value at this time, as long as it is clear that the bacteria can also be put into the tank.
3. For the fermentation liquid without extraction value, it should be heated to above 120 °C before being discarded and kept for 30 minutes before discharge.
Fourth, the rescue plan for the equipment after fermenting the tank
Before the fermenter is reused after dyeing, it must be thoroughly cleaned after the can, and the empty can is heated and sterilized to above 120 °C for 30 minutes before use. It can also be treated by formaldehyde fumigation or soaking in formaldehyde solution for more than 12 hours.
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