1. Appearance Appearance and identification of the appearance of the species, the mycelium is thick white, thick and elastic, and the vitality is strong; if the mycelium of mycelia grows atrophy, is dry and has no color, or the mycelium autolysis produces a lot of red brown liquid, then life The force has become weaker and should not be reused. If the wooden sclerotinium species are still kept hard, it belongs to the strain of strong living force. If the wood blocks become soft and loose, they are already aging and should not be used. 2. Observation and identification of culture For the strains isolated, selected and introduced, the adaptation characteristics of mycelium to dryness, humidity and temperature were observed by culturing. If the mycelium is cultured under conditions of partial dryness, partial moisture, and wet and dry conditions, if the mycelium grows well under the first two conditions, and grows best under the wet and dry conditions, then it is good. Strains. 3, liquid culture identification Preparation of 2% aqueous sugar solution, after conventional sterilization, picking out the large bean seed mass, into 100 ml of the above solution, placed at 25 - 28 °C temperature culture 3 ~ 7 days, If there is a bubble on the liquid surface, “oil skin†occurs and turbidity occurs, indicating that the strain itself has miscellaneous bacteria; if the seedling block sinks, or if a thin layer of mycelium grows later, it indicates that the viability of the strain is weak; If the mycelial growth around the liquid surface is fast, and the thick white is cotton-like, it indicates that the species has a strong vitality. 4ã€Sawdust bottle identification and cycle yield identification Bottle cultivation identification practices are similar to those of cultivating cultivation methods. Sawdust cultivating material is loosened, humidity is increased, and the required bacteria species are inoculated into the culture medium. Record well and incubate at a constant temperature of 26°C for 15 days. Then the temperature was reduced to 15-20 °C, and given a better condition of scattered light, and then cultivated for about half a month, fruit body primordium and a few fruiting bodies appeared on the wall and the material surface. If no bacteria and abnormalities are found, the strains will be attached to the wood section and the cycle yield identification will be conducted. If the fruiting body grows vigorously, high-yield and high-quality, with excellent cultivar characteristics, and no miscellaneous bacteria are mixed, indicating that the strain is reliable, it can be preserved and put into large-scale production. Practice has proved that the identification methods for the quality of the above strains are more scientific and can screen out fine strains for the majority of users.
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